Active Rab5

Active Rab5
Catalog Number: 26911
Gene Symbol: RAB5

Description: Anti-Active Rab5 Mouse Monoclonal Antibody

Background: Rab5 is a member of Rab-family
GTPases. Rab5 is a rate-limiting component of the
machinery regulating the kinetics of membrane
traffic in the early endocytic pathway.
Immunogen: Recombinant full length protein of
active Rab5A
Tested applications: IP, IHC
Recommended dilutions:
1 μg for ~2 mg total cellular proteins for IP;
1:100 dilution for IHC
Concentration: 1 mg/ml
Host: mouse
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Format: Liquid
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH
7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-active Rab5 antibody
recognizes active Rab5 proteins of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze
/ thaw cycles

Rab5 activation assay. Purified full-length
Rab5A proteins were immunoprecipitated
after treated with GDP (lane 1) or GTPγS
(lane 2). Immunoprecipitation was done
with the anti-active Rab5 monoclonal
antibody (Cat. No. 26911). Immunoblot was
with an anti-Rab5 polyclonal antibody (Cat.
No. 21151).