Active Arf6

Active_Arf6
Catalog Number: 26918
Gene Symbol: ARF6

Deion: Anti-Active Arf6 Mouse Monoclonal Antibody

Background: ARF6 (ADP-ribosylation factor 6)
is a member of the ARF super-family. ARF genes
encode small GTPases that increase the
ADP-ribosyltransferase activity of cholera toxin
and are critical for vesicular trafficking as
activators of phospholipase D. Arf6 regulates
membrane trafficking and functions as a
regulatory molecule of phagocytosis.
Immunogen: Recombinant full length protein of
active Arf6.
Tested applications: IP, IHC
Recommended dilutions:
IP: 1 μg for 1~2 mg total cellular proteins
IHC: 1:50~100
Concentration: 1 mg/ml
Host: mouse
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Format: Liquid
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH
7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-active Arf6 antibody
recognizes active Arf6 from vertebrates.
Storage Conditions: Store at -20°C. Avoid
freeze / thaw cycles

Arf6 activation assay. Purified full-length Arf6
proteins were immunoprecipitated after treated
with GDP (lane left) or GTPγS (lane right).
Immunoprecipitation was done with the
anti-active Arf6 monoclonal antibody (Cat. No.
26918). Immunoblot was with an anti-Arf6
polyclonal antibody (Cat. No. 21032).