RhoA antibody

Active RhoA
Catalog Number: 26904
Gene Symbol: RHOA
Description: Anti-Active RhoA Mouse Monoclonal Antibody

Background: Small GTPases are a super-family
of cellular signaling regulators. RhoA belongs to
the Rho sub-family of GTPases. Rho proteins
play critical roles in many actin cytoskeletondependent
processes including platelet
aggregation, cell motility, contraction, and
cytokinesis. It regulates the formation of stress
fibers and focal adhesions in fibroblasts and Ca2+
sensitivity of smooth muscle contraction.
Immunogen: Recombinant full length protein of
active RhoA
Tested applications: IP, IHC
Recommended dilutions:
1 μg for 1~2 mg total cellular proteins
Concentration: 1 mg/ml
Host: mouse
Clonality: Monoclonal
Isotype: IgG2b, IgM
Purity: Purified from ascites
Format: Liquid
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH
7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-active RhoA antibody
recognizes active RhoA from vertebrates.
Storage Conditions: Store at -20°C. Avoid
freeze / thaw cycles

IP/WB analysis of active RhoA protein. Purified
recombinant RhoA proteins were loaded with
GDP (lane 1) or GTPgS (lane 2). These proteins
were immunoprecipitated with anti-active RhoA
mouse monoclonal antibody (Cat. # 26904).
After SDS/PAGE, the membrane filter was
probed with anti-RhoA mouse monoclonal
antibody (Cat. # 26007).