生物发酵罐Biological Fermentor
发酵罐概述Overview Of Fermentation Tank
常见的发酵罐分为固体发酵罐和液体发酵罐。发酵罐根据容积和用途不同，分为种子罐（菌种发酵罐）和发酵罐。发酵罐根据需氧类型的不同又分为通风式发酵罐和厌氧发酵罐。发酵罐、种子罐，有碳钢制、不锈钢、高镍基合金等，主要用于生化发酵生产。如生产味精、柠檬酸、酶制剂、溶剂、制药、食品等行业。
Common fermentation tanks are divided into solid fermentation tank and liquid fermentation tank. Fermentation tank is divided into seed tank (strain fermentation tank) and fermentation tank according to different volume and purp o se. According to different aerobic types, fermentation tanks are divided into ventilated fermentation tanks and anaerobic fermentation tanks. Fermentation tank and seed tank, made of carbon steel, stainless steel and high nickel base alloy, are mainly used for biochemical fermentation production. Such as monosodium glut a mate, citric acid, enzyme preparation, solvent, pharmaceutical, food and other industries.
一、发酵罐的基本条件Basic Conditions Of Fermentation Tank
机械搅拌发酵罐是发酵工厂常用类型之一。它是利用机械搅拌器的作用，使空气和醪液充分混合，促使氧在醪液中溶解，以保证供给微生物生长繁殖、发酵所需要的氧气。使用发酵罐的基本要求：
Mechanical stirring fermentor is one of the commonly used types in fermentation plants. It uses the function of mechanical stirrer to fully mix air and mash, and promote the dissolution of oxygen in mash, so as to ensure the supply of oxygen required for microbial growth, reproduction and fermentation. Basic requirements for using fermentor:
1、发酵罐应具有适宜的径高比。发酵罐的高度与直径之比一般为1.7~4倍左右，罐身越长，氧的利用率较高。
2、发酵罐能承受一定压力。由于发酵罐在消毒及正常工作时，罐内有一定的压力（气压与液压）和温度，因此罐体各部件要有一定的强度，能承受一定的压力。
3、发酵罐的搅拌通风装置使气液充分混合，保证发酵液必须的溶解氧。
4、发酵罐应具有足够的冷却面积。微生物生长代谢过程放出大量的热量，为了控制发酵过程不同阶段所需的温度，应装有足够的冷却面积。
5、发酵罐内应尽量减少死角，避免藏垢积污，灭 菌能彻 底，避免染菌。
6、搅拌器的轴封应严密，尽量减少泄漏。
1、The fermentation tank shall ha ve appropriate diameter height ratio. The ratio of height to diameter of fermentation tank is generally about 1.7 ~ 4 times. The longer the tank body, the higher the utilization rate of oxygen.
2、The fermentation tank can withstand certain pressure. Since there is a certain pressure (air pressure and hydraulic pressure) and temperature in the fermentation tank during disinfection and normal operation, all parts of the tank must ha ve a certain strength and can withstand a certain pressure.
3、The stirring and ventilation device of the fermentation tank can fully mix the gas and liquid to ensure the dissolved oxygen of the fermentation broth.
4、The fermentation tank shall ha ve sufficient cooling area. Microbial growth and metabolism process releases a lot of heat. In order to con t rol the temperature required in different stages of fermentation process, sufficient cooling area should be installed.
5、The dead corner shall be minimized in the fermentation tank to a void dirt accumulation. The sterilization can be complete and bacteria cont a mination can be a voided.
6、The shaft seal of the mixer shall be tight to minimize leakage.
二、发酵罐罐体尺寸比例Size Proportion Of Fermentation Tank
● 罐体各部分的尺寸有一定的比例，罐的高度与直径之比一般为1.7~4倍左右。
● 发酵罐通常装有两组搅拌器，两组搅拌器的间距S约为搅拌器直径的三倍。
● 对于大型发酵罐以及液体深度HL较高的，可安装三组或三组以上的搅拌器。
● z u i下面一组搅拌器通常与风管出口较接近为好，与罐底的距离C一般等于搅拌器直径Di，但也不宜小于0.8Di，否则会影响液体的循环。
● The size of each part of the tank body has a certain proportion, and the ratio of tank height to diameter is generally about 1.7 ~ 4 times.
● The fermentation tank is usually equipped with two groups of agitators, and the spacing S between the two groups of agitators is about three times the diameter of the agitator.
● For large fermentation tanks and those with high liquid depth HL, three or more groups of agitators can be installed.
● The bottom group of agitators is usually close to the outlet of the air duct. The distance C from the tank bottom is generally equal to the agitator diameter Di, but it should not be less than 0.8di, otherwise the liquid circulation will be affected.
三、发酵罐的结构Structure Of Fermentation Tank
好气性机械搅拌发酵罐是密封式受压设备，主要部件包括罐身、搅拌器、轴封、打泡器、联轴器、中间轴承、空气吹泡管（或空气喷射器）、挡板、冷却装置、人孔以及管路等。
系统由发酵罐，空气处理系统、蒸汽净化系统、电器控制系统、恒温系统及管路、阀门、辅助系统，传感器与一次仪表系统，下位机控制系统（现场PLC控制系统，二次仪表），上位机系统以及台架等组成。
Aerobic mechanical stirring fermentation tank is a sealed pressure equipment, and its main components include tank body, agitator, shaft seal, bubbler, coupling, intermediate bearing, air bubbling pipe (or air ejector), baffle, cooling device, manhole and pipeline.
The system is comp o sed of fermentation tank, air treatment system, steam purification system, electrical con t rol system, thermostatic system and pipeline, valve, auxiliary system, sensor and primary instrument system, lower computer con t rol system (field PLC con t rol system, secondary instrument), upper computer system and bench.
技术参数/Technical Parameter
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公称体积 Nominal Volume
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5~3000L
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装液系数
Liquid Loa d ing Coefficient
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65-80%, z u i佳 Optimum 70%
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罐体配置
Can Body Configuration
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不锈钢SUS316L/SUS304材质；
夹套控温，优化导流设计；
内表面抛光，Ra≤0.6μm；外表面抛光或亚光处理（玻璃喷丸）；
大视角长条视镜，pH电极、DO电极、温度电极接口齐备；
罐盖配有火焰接种口和补料备用接口等
Stainless steel SUS316L / SUS304 Material;
Jacket temperature con t rol and optimization of diversion design;
Internal surface polishing, RA ≤ 0.6 μ m； External surface polishing or matte treatment (glass shot blasting);
Long strip mirror with large viewing angle, complete interfaces of pH electrode, do electrode and temperature electrode;
The tank cover is equipped with flame inoculation port and feeding standby interface, etc
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搅拌系统
Mixing System
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顶入式机械搅拌系统；无菌机封系统；直流搅拌电机（10L及以下）或交流电机；
标配2层平直叶轮，一级高 效消泡桨；另有斜叶、弯叶、轴向流桨叶等可选；
可调速，数字化设定转速控制
Jacking mechanical mixing system; Aseptic mechanical sealing system; DC mixing motor (10L and below) or AC motor;
Standard configuration: 2-layer flat impeller and one-stage high-efficiency defoaming paddle; There are also inclined blades, curved blades, axial flow blades, etc;
Adjustable speed, digital setting speed con t rol
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结构方式
Structure Mode
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落地式框架结构，节省空间，万向脚轮，操作方便
Floor type frame structure, space sa ving, universal c a sters, convenient operation
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通气系统
Ventilation System
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深层通气，全不锈钢管路；
玻璃转子流量计显示，压力表显，手动阀门调节流量；
配备不锈钢外壳的高 效除 菌过滤器，过滤精度0.01μm；
需外源压缩空气，配备优 质除水稳压装置，持续稳定供气；
可选配质量流量控制器自动控制空气流量
Deep ventilation, all stainless steel pipeline;
Glass rot a meter display, pressure gauge display, manual valve to adjust flow;
Equipped with high-efficiency sterilization filter with stainless steel shell, the filtration accuracy is 0.01 μm；
External compressed air is required, and high-quality water removal and pressure stabilizing device is equipped to continuously and stably supply air;
Optional mass flow con t roller to automatically con t rol air flow
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罐压控制
Tank Pressure Con t rol
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顶部排气口配置指针式压力表显示罐压，不锈钢阀门手动调节；
可选配进口压力变送器和自动调节阀实现罐压自动控制
The top exhaust port is equipped with a pointer pressure gauge to display the tank pressure, and the stainless steel valve is manually adjusted;
It can be equipped with inlet pressure transmitter and automatic regulating valve to realize automatic con t rol of tank pressure
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温度控制
Temperature Con t rol
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在线检测，数字化设定，自动/手动控制自由切换；
德国Pt-100温度探头和数字式温度仪表；
电加热恒温水箱与外源冷却水自动控制温度
Online detection, digital setting, automatic / manual con t rol, free switching;
German PT-100 temperature probe and digital temperature instrument;
Automatic temperature con t rol of electric heating thermostatic water tank and external cooling water
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Ph控制
Ph Con t rol
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在线检测，1路或2路蠕动泵自动流加碱或酸液进行调节；
进口梅特勒（汉密尔顿）pH电极，可高温蒸汽消毒
Online detection, 1 or 2 peristaltic pumps automatically add alkali or acid for regulation;
Imported Mettler (Hamilton) pH electrode, which can be sterilized by high temperature steam
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溶氧(Do)
Dissolved Oxygen(Do)
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在线检测，范围0-1 0 0 %或0-2 0 0 %；
进口梅特勒（汉密尔顿）DO电极，可高温蒸汽消毒；
可选与转速、通气量联动控制（订购时需明确）
Online detection, range 0-1 0 0 % or 0-2 0 0 %;
Imported Mettler (Hamilton) do electrode, which can be sterilized by high temperature steam;
Optional linkage con trol with speed and ventilation (to be specified when ordering)
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补料控制
Replenishment Con t rol
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标配1路/2路等多路蠕动泵时间比例流加补料，流加量累积显示、记录
It is equipped with 1-way / 2-way and other multi-channel peristaltic pumps as standard, with time proportional flow feeding and feeding, and the cumulative flow feeding amount is displayed and recorded
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消泡方式
Defoaming Mode
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传导式泡沫电极，泡沫异常状况自动报警；
标配1路蠕动泵自动/手动流加消泡剂，流加量累积显示、记录
The conductive foam electrode automatically alarm the abnormal condition of foam.
One peristaltic pump is equipped as standard for automatic / manual flow of defoamer, and the cumulative flow dosage is displayed and recorded
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控制系统
Con trol System
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应用工业级MC-bio控制系统，采用西 门 子PLC控制器；
32位真彩色触摸屏操作界面，功能齐全、运行稳定；
另有A型简易型分仪表控制系统可选
Apply industrial MC bio con trol system and Siemens PLC con troller;
32-bit true color touch screen operation interface with complete functions and stable operation;
Another type a simple instrument con trol system is optional
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灭 菌 方式 Sterilization Method
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在位灭 菌，手动控制，需接外源饱和蒸汽
In place sterilization, manual con trol, need to connect external saturated steam
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可选配项
Optional Configuration
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1.多路补料Multiple feeding
2.全自动灭 菌Automatic sterilization(AUTO-SIP)
3.补料称重Feeding and weighing
4.罐体称重Tank weighing
5.富氧旁路Oxygen enriched bypass
6.氧化还原电位在线检测On line detection of redox potential
7.空气流量自动检测与控制Automatic detection and con trol of air flow
8.尾气CH4，O2、CO2含量在线检测Online detection of CH4, O2 and CO2 content in tail gas
9.甲 醇（乙 醇）含量在线检测Online detection of methanol (ethanol) content
10.葡萄糖、特定氨基酸在线检测On line detection of glucose and specific amino acids
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设备特点
Equipment Characteristics
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在位灭 菌，安 全可靠，培养基浓度准确；
无传动泄漏风险；
大视角纵向视镜观测清晰；
接种方式多样可靠（标配火焰接种口，差压接种口等多种形式可选）；
落地式罐型美观大方；
操作拆装容易；
特殊规格可以定制。
In situ sterilization, safe and reliable, accurate medium concen tration;
No risk of transmission leakage;
Large viewing angle longitudinal mirror observation is clear;
Various and reliable inoculation methods (standard flame inoculation port, differential pressure inoculation port and other forms are optional);
Floor type tank, beautiful and generous;
Easy operation and disassembly;
Special specifications can be customized.
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