IDH2(R172H) 小鼠单抗

厂商 ：武汉费斯德生物科技有限公司

湖北 武汉市
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商品详细描述
货号:   26084
基因符号:   IDH2
描述:   Anti-IDH2(R172H) Mouse Monoclonal Antibody
背景介绍:   Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-172 in IDH2 to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.
免疫原:   A synthetic peptide from the internal region of human IDH2, which includes the mutation of R172H
经过测试的应用:   ELISA, WB, IHC, IF
推荐稀释比:    ELISA: 1: 1000-1: 5000 WB: 1: 100-1: 1000 IHC: 1: 50-1: 100 IF: 1: 50-1: 100
浓度:   1 mg/ml
宿主:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯度:   Purified from ascites
防腐剂: No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Anti-IDH2(R172H) antibody recognizes IDH2(R172H) of vertebrates
储存条件:   Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:   Western blot analysis of recombinant IDH2(R172H) and wild-type IDH2 proteins. Purified his-tagged IDH2(R172H) protein (Cat. # 10201) and wild-type IDH2 protein (Cat. # 10200) were blotted with anti-IDH2(R172H) monoclonal antibody (Cat. # 26084) (left panel). Proteins loading were blotted with anti-IDH2 monoclonal antibody (right panel). 免疫荧光:   Immunofluorescence of cells expressing IDH2 proteins with Anti-IDH2(R172H) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2(R172H) plasmid (right column), then fixed and stained with Anti-IDH2(R172H) monoclonal antibody (Cat. #26084).
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