BRAF(N581S) 小鼠单抗

厂商 ：武汉费斯德生物科技有限公司

湖北 武汉市
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商品详细描述
货号:   26109
基因符号:   BRAF, BRAF1, RAFB1
描述:   Anti-BRaf(N581S) Mouse Monoclonal Antibody
背景介绍:   B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
免疫原:   A synthetic peptide from the internal region of Braf (N581S), human origin
经过测试的应用:   ELISA, WB, IHC, IP
推荐稀释比:   
ELISA: 1: 1000-1: 5000
WB: 1: 500-1: 2000
IHC: 1: 100-200
浓度:   0.5 mg/ml
宿主:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯度:   Purified from ascites
防腐剂: No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Recognizes B-Raf N581S, but not wild-type B-Raf proteins from vertebrates
储存条件:   Store at -20°C. Avoid repeated freezing and thawing

蛋白质印迹:   

Western blot analysis of recombinant B-raf proteins.

Purified His-tagged BRaf(N581S) protein (amino acids 513-693, lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-BRaf(N581S) monoclonal antibody (Cat. # 26109).

免疫荧光:   

Immunofluorescence of cells expressing B-Raf proteins with Anti-BRaf(N581S) antibody. HEK293T cells were transfected with pCDNA3-GFP-B-Raf (WT) plasmid (left column) or pCDNA3-GFP-BRaf(N581S) plasmid (right column), then fixed and stained with Anti-BRaf(N581S) monoclonal antibody (Cat. #26109).

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