BRaf 多抗

厂商 ：武汉费斯德生物科技有限公司

湖北 武汉市
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联系电话 ：18162422833
商品详细描述
     
货号:   21271
基因符号:   B-RAF, B-RAF1, RAFB1
描述:   Anti-BRaf Rabbit Polyclonal Antibody
背景介绍:   B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the B-RAF gene cause diseases. Inherited mutations in B-RAF cause cardiofaciocutaneous syndrome. Acquired mutations in B-RAF have been found in cancers.
免疫原:   Purified B-Raf protein, human origin
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   
ELISA: 1: 1000-1: 5000
WB: 1: 100-1: 1000
IHC: 1: 50-1: 200
浓度:    0.1mg/ml
宿主:   Rabbit
形式:   Liquid
克隆性:   Polyclonal
纯度:   Purified from serum
防腐剂: No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Recognizes B-Raf of vertebrates
储存条件:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry:   

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with anti- B-Raf polyclonal antibody (Cat. #21271). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1: 50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1: 50 dilution) was used as secondary antibody.

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