Arl1-GTP 小鼠单抗

厂商 ：武汉费斯德生物科技有限公司

湖北 武汉市
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商品详细描述
货号:   26924
规格:   30 ?L
基因符号:   Arl1
描述:   Anti-Arl1-GTP Mouse Monoclonal Antibody
背景介绍:   Arl1 (Arf-like 1) localizes to the Golgi complex, where it is believed to recruit effector proteins to the trans-Golgi network. Golgi-localized proteins golgin-97 and golgin-245 have been shown as Arl1 effectors. Additional Arl1 effectors include the GARP (Golgi-associated retrograde protein)/VFT (Vps53) vesicle-tethering complex and Arfaptin 2. In Drosophila zygotes, mutation of Arl1 is lethal.
免疫原:   Recombinant Arl1 protein (active form)
经过测试的应用:   IP, IHC, IF
推荐稀释比:   
IP: 1 ?g for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250
浓度:   1 mg/ml
宿主:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯度:   Purified from ascites
防腐剂:   no
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Arl1 antibody recognizes active Arl1-GTP from vertebrates.
储存条件:   Store at -20°C. Avoid repeated freezing and thawing
免疫沉淀/蛋白质印迹:   

Arl1 Activation Assay. Purified GST-tagged Arl1 proteins (Cat. #10136) were immunoprecipitated with the anti-active Arl1 monoclonal antibody (Cat. # 26924) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti- Arl1 monoclonal antibody (Cat. #26056). Arl1 protein input control is shown in the bottom panel.

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