Cdc42-GTP 小鼠单抗

厂商 ：武汉费斯德生物科技有限公司

湖北 武汉市
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联系电话 ：18162422833
商品详细描述
货号:   26905
规格:   30 ?L
基因符号:   CDC42
描述:   Anti-Cdc42-GTP Monoclonal Antibody
背景介绍:   Small GTPases are a super-family of cellular signaling regulators. Cdc42 belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene tranion. GTP binding increases the activity of Cdc42, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).
免疫原:   Recombinant full length protein of active Cdc42
经过测试的应用:   IP, IHC
推荐稀释比:   
IP: 1 ?g for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250
浓度:   1 mg/ml
宿主:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG1
纯度:   Purified from ascites
防腐剂:   No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Anti-Cdc42-GTP monoclonal antibody recognizes active Cdc42 from vertebrates.
储存条件:   Store at -20°C. Avoid repeated freezing and thawing

IP-蛋白质印迹:   

IP/WB analysis of active Cdc42 protein. Purified recombinant Cdc42 proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-Cdc42-GTP mouse monoclonal antibody (Cat. # 26905). After SDS/PAGE, the membrane filter was probed with anti-Cdc42 mouse monoclonal antibody (Cat. # 26008).

免疫荧光:

Immunofluorescent labeling of Cdc42-GTP in MS1 mouse endothelial cells. The MS1 cells cultured on glass coverslips (Fisher) in DMEM containing 5% FBS were fixed with 4% paraformaldehyde for 10 minutes at RT (room temperature) and permeabilized in PBSN (PBS+0.1%NP40) for 15 minutes at RT. The cells were stained with primary antibody (Cdc42-GTP) at 1: 25 dilution in Invitrogen’s CAS-BLOCK (00-8120) reagent at RT for 1 hour. After brief washing in PBSN (3x5min), a secondary Alexa555 conjugated goat anti mouse antibody (Invitrogen A21424) was applied in 1: 200 in CAS-BLOCK at RT for 1 hour. The coverslips were then washed in PBSN (3x5min) and mounted using vectorsheld’s hard set mounting medium (H-1500), and examined using a Zeiss inverted fluorescence microscope.

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