Gαi-GTP 小鼠单抗

厂商 ：武汉费斯德生物科技有限公司

湖北 武汉市
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联系电话 ：18162422833
商品详细描述
货号:   26901
基因符号:   Gnai
描述:   Anti-Gαi-GTP Mouse Monoclonal Antibody
背景介绍:   Heterotrimeric G proteins are critical cellular signal transducers. Gαi represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gα i proteins are being explored.
免疫原:   Recombinant full length protein of active Gα i1
经过测试的应用:   IP, IHC, IF
推荐稀释比:   
IP: 1 ?g for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250
浓度:   1 mg/ml
宿主:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG1
纯度:   Purified from ascites
防腐剂:   No
成分:   PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Anti-active Gα i antibody recognizes active Gα i1, Gα i2, and Gα i3 of vertebrates
储存条件:   Store at -20°C. Avoid repeated freezing and thawing
免疫沉淀/蛋白质印迹:   
Gαi activation assay.
A. CHO cells were transfected with wild-type Gα i1 (lanes 1 and 2) or constitutively active Gα i1-Q204L (lane 3). Cell lysates were treated with GDP(lane 1) or GTPγS (lane 3). Lysates were then incubated with an anti-active Gα i monoclonal antibody (Cat. No.26901) (top panel). The precipitated active Gα i was immunoblotted with an anti-Gα i monoclonal antibody (Cat. No. 26003). The bottom panel shows the Western blot with anti-Gα i monoclonal antibody (Cat.No. 26003) of the cell lysates.
B. HEK293 cells stably expressing human m2 mAChR were treated with (lane 2) or without (lane 1)carbachol. Cell lysates were then incubated with an anti-active Gα i monoclonal antibody (Cat. No. 26901) (top panel).The precipitated active Gα i was immunoblotted with an anti-Gα i rabbit polyclonal antibody (Cat. No. 21006). The bottom panel shows the Western blot with anti-tubulin of the cell lysates.
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