BRaf(V600E)点突变抗体

厂商 ：武汉纽斯特生物技术有限公司

湖北 武汉市
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  • 蛋白点突变
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商品详细描述

BRaf(V600E) [26039]
[100 ?L]


Catalog Number:  26039
Gene Symbol:  BRaf
Description:   Anti BRaf(V600E) Mouse Monoclonal Antibody
Background:   BRaf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers
Immunogen:   A synthetic peptide from the internal region of BRaf(V600E), human origin.
Applications:   ELISA, WB, IHC, IP
Recommended Dilutions:   
ELISA: 1:100-1:5000 
WB: 1:100-1:2000 
IHC: 1:100-1:1000
Concentration:   0.5 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG2b
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes BRaf(V600E), but not wild type BRaf proteins from vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
Download Datasheet:  
Western blot:  
Western blot analysis of recombinant Braf proteins with anti-BRaf(V600E) antibody. Purified His - tagged BRaf(V600E ) ?amino acids 513-693)(lane1) ? corresponding wild type protein(lane 2), His - tagged BRaf(V600D ) (lane 3) , His - tagged BRaf(V600A ) (lane 4) , His - tagged BRaf(V600G ) (lane 5) , His - tagged BRaf(V600K ) (lane 6), His - tagged BRaf(V600R ) (lane 7), and His - tagged BRaf( V600M ) (lane 8) were blotted with anti BRaf(V600E) monoclonal antibody (Cat # 26039) .
Immunofluorescence:  
Immunofluorescence of cell expressing BRaf protein with anti BRaf(V600E) antibody . HEK293T cells were transfected with pCDNA3-GFP BRaf(V600E )plasmid,pCDNA3-GFP BRaf( WT )plasmid, pCDNA3-GFP BRaf(V600K )plasmid,pCDNA3-GFP BRaf(V600R )plasmid, pCDNA3-GFP- BRaf(V600A )plasmid,pCDNA3-GFP BRaf(V600D ) plasmid, pCDNA3-GFP-BRaf(V600M)plasmid, or pCDNA3-GFP-B-Raf(V600G)plasmid, then fixed and stained with anti BRaf(V600E) monoclonal antibody (Cat. # 26039).
Immunohistochemistry:   
Immunohistochemical analysis of paraffin-embedded human melanoma tissue with anti BRaf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:100) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1:50) was used as secondary antibody.


Related Products
Catalog# Name Size Price
21271 Anti BRaf Rabbit Polyclonal Antibody  100 μL  ￥2600   
26129 Anti BRaf Mouse Monoclonal Antibody  100 μL  ￥2800 
26109 Anti BRaf(N581S) Mouse Monoclonal Antibody  100 μL  ￥4800
26039 Anti BRaf(V600E) Mouse Monoclonal Antibody  100 μL  ￥4800
26110 Anti BRaf(V600K) Mouse Monoclonal Antibody  100 μL  ￥4800
26184 Anti BRaf(V600D) Mouse Monoclonal Antibody  100 μL  ￥4800
26182 Anti BRaf(V600R) Mouse Monoclonal Antibody  100 μL  ￥4800
26196 Anti BRaf(G469E) Mouse Monoclonal Antibody  100 μL  ￥4800

26199 Anti BRaf(V600A) Mouse Monoclonal Antibody  100 μL  ￥4800
26462 Anti BRAF(V600M) Mouse Monoclonal Antibody  100 μL  ￥4800



Publications:
1.  Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
    Mod Pathol. 2013 Mar;26(3):414-20.
2.  Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
    Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733
3.  Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
    human pathology. February 2015Volume 46, Issue 2, Pages 169–175
4.  Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
    human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570
5.  BRAF V600E Mutation-Specific Antibody: A Review

    Seminars in Diagnostic Pathology

6.Reactivation of ERK and Akt confers resistance of mutant BRAF colon cancer cells to the HSP90 inhibitor AUY922

    Oncotarget. 2016 Aug 2; 7(31): 49597–49610.

7.PES1 promotes the occurrence and development of papillary thyroid cancer by upregulating the ERα/ERβ protein ratio

   Sci Rep. 2019; 9: 1032.

8.Targeting of the MAPK and AKT pathways in conjunctival melanoma shows potential synergy

    Oncotarget. 2017 Aug 29; 8(35): 58021–58036

9.Clinical, Histologic, and Immunophenotypic Features of Serrated Polyps in Patients With Inflammatory Bowel Disease

    Gastroenterology Res. 2018 Oct; 11(5): 355–360

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