Active Gαi

厂商 ：武汉纽斯特生物技术有限公司

湖北 武汉市
  • 主营产品：
  • G蛋白活性
  • cAMP和cGMP ELISA试剂盒
  • 蛋白点突变
联系电话 ：15002729010
商品详细描述

ctive Gαi GTP [26901]
[30 ?L]


Catalog Number:  26901
Gene Symbol:  Gnai
Description:   Anti Active Gαi Mouse Monoclonal Antibody
Background:   Heterotrimeric G proteins are critical cellular signal transducers. Gαirepresents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαi proteins are being explored.
Immunogen:   Recombinant full length protein of active Gαi1
Applications:   IP, IHC
Recommended Dilutions:   1 ?g for 1~2 mg total cellular proteins for IP; 
                                                   1:100 dilution for IHC
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG1
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti active Gαi antibody recognizes active Gαi1, Gαi2, and Gαi3 of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
Download Datasheet:  
Immunoprecipitation/Western blot:  
Gαi activation assay. 
A. CHO cells were transfected with wild-type Gαi1 (lanes 1 and 2) or constitutively active Gαi1-Q204L (lane 3). Cell lysates were treated with GDP(lane 1) or GTPγS (lane 3). Lysates were then incubated with an anti-active Gαi monoclonal antibody (Cat. No.26901) (top panel). The precipitated active Gαi was immunoblotted with an anti Gαi monoclonal antibody (Cat. No. 26003). The bottom panel shows the Western blot with anti Gαi monoclonal antibody (Cat.No. 26003) of the cell lysates. 
B. HEK293 cells stably expressing human m2 mAChR were treated with (lane 2) or without (lane 1)carbachol. Cell lysates were then incubated with an anti active Gαi monoclonal antibody (Cat. No. 26901) (top panel).The precipitated active Ga i was immunoblotted with an anti Gαi rabbit polyclonal antibody (Cat. No. 21006). The bottom panel shows the Western blot with anti tubulin of the cell lysates.


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