BRaf(V600E) 2603厂家、批发、价格、电话

厂商：武汉纽斯特生物技术有限公司

湖北武汉市

主营产品：
G蛋白活性
cAMP和cGMP ELISA试剂盒
蛋白点突变

联系电话：15002729010

商品详细描述

价格	￥4800.00
品牌	NewEastBio
产地	美国
货号	26039
免疫原	Mouse
规格	100ul

BRaf(V600E) [26039]
[100 ?L]

Catalog Number: 26039

Gene Symbol: BRaf

Description: Anti BRaf(V600E) Mouse Monoclonal Antibody

Background: BRaf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers

Immunogen: A synthetic peptide from the internal region of BRaf(V600E), human origin.

Applications: ELISA, WB, IHC, IP

Recommended Dilutions:
ELISA: 1:100-1:5000
WB: 1:100-1:2000
IHC: 1:100-1:1000

Concentration: 0.5 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG2b

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes BRaf(V600E), but not wild type BRaf proteins from vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

Western blot analysis of recombinant Braf proteins with anti-BRaf(V600E) antibody. Purified His - tagged BRaf(V600E ) ?amino acids 513-693)(lane1) ? corresponding wild type protein(lane 2), His - tagged BRaf(V600D ) (lane 3) , His - tagged BRaf(V600A ) (lane 4) , His - tagged BRaf(V600G ) (lane 5) , His - tagged BRaf(V600K ) (lane 6), His - tagged BRaf(V600R ) (lane 7), and His - tagged BRaf( V600M ) (lane 8) were blotted with anti BRaf(V600E) monoclonal antibody (Cat # 26039) .

Immunofluorescence:

Immunofluorescence of cell expressing BRaf protein with anti BRaf(V600E) antibody . HEK293T cells were transfected with pCDNA3-GFP BRaf(V600E )plasmid,pCDNA3-GFP BRaf( WT )plasmid, pCDNA3-GFP BRaf(V600K )plasmid,pCDNA3-GFP BRaf(V600R )plasmid, pCDNA3-GFP- BRaf(V600A )plasmid,pCDNA3-GFP BRaf(V600D ) plasmid, pCDNA3-GFP-BRaf(V600M)plasmid, or pCDNA3-GFP-B-Raf(V600G)plasmid, then fixed and stained with anti BRaf(V600E) monoclonal antibody (Cat. # 26039).

Immunohistochemistry:

Immunohistochemical analysis of paraffin-embedded human melanoma tissue with anti BRaf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:100) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1:50) was used as secondary antibody.

Publications:

1. Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
Mod Pathol. 2013 Mar;26(3):414-20.

2. Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733

3. Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
human pathology. February 2015Volume 46, Issue 2, Pages 169–175

4. Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570

5. BRAF V600E Mutation-Specific Antibody: A Review

Seminars in Diagnostic Pathology

6.Reactivation of ERK and Akt confers resistance of mutant BRAF colon cancer cells to the HSP90 inhibitor AUY922

Oncotarget. 2016 Aug 2; 7(31): 49597–49610.

7.PES1 promotes the occurrence and development of papillary thyroid cancer by upregulating the ERα/ERβ protein ratio

Sci Rep. 2019; 9: 1032.

8.Targeting of the MAPK and AKT pathways in conjunctival melanoma shows potential synergy